Invitrogen™ SPHK2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA585212

Catalog No. PIPA585212

Description

Keep as concentrated solution. Predicted reactivity: Mouse (89%), Rat (89%), Rhesus Monkey (97%). Positive Control: PC-3, U87-MG. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

SPHK2, along with SPHK1, catalyzes the phosphorylation of sphingosine producing the signaling molecule sphingosine-1-phosphate (S1P). S1P signals through G-protein coupled receptors and controls diverse cellular processes. Inhibitors of SPHKs are viewed as potential therapeutics for the treatment of cancer. SPHK2 is an important signaling molecule with intra- and extracellular functions and has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors). These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide raange of cellular events including cell motility, survival, apoptosis, migration and cell-cell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).

Specifications

• Antigen: SPHK2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Sphk2
• Gene Alias: C76851; SK 2; SK2; SK-2; Sphingosine kinase 2; sphingosine kinase type 2; Sphk2; SPK 2; SPK-2; Spk2, Sk2
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 56848
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q9NRA0
• Gene Symbols: Sphk2
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SPHK2.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG