SPI-B Monoclonal Antibody (235D), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
14-979-082	Invitrogen™ 14979082	100 μg
14-979-037	Invitrogen™ 14979037	2 mg

Description

Description: The 235D monoclonal antibody recognizes human SPI-B. SPI-B is a member of the ETS transcription factor family which is comprised of DNA binding proteins that function in lymphoid development and activity. SPI-B binds the PU box, a purine-rich DNA sequence (5'-GAGGAA-3'). SPI-B is expressed in mature B cells, immature T cells, plasmacytoid dendritic cells, and microfold cells (M cells) of the intestine. In human tonsil tissue, high levels of SPI-B are expressed in plasmacytoid dendritic cells, while lower levels of expression are found in germinal center B cells. Applications Reported: This 235D antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This 235D antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Spi-B is a transcription factor belonging to a subfamily of ETS transcription factors with a conserved ETS domain that mediates specific DNA binding. Spi-B may form homotypic interactions and is known to interact with IRF4, CREBBP, EP300, SPI1/PU.1 related, JUN and TBP. As a sequence specific transcriptional activator it binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Two isoforms of Spi-B are reported. Spi-B is usually expressed in plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells and promotes their development and block viral replication. Spi-B is not expressed in T-cells or granulocytes but may be present in stem cell populations of the liver and B-cells where it helps in B-cell receptor (BCR) signaling, normal B-cell development and antigenic stimulation.

Specifications

• Antigen: SPI-B
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: B42b-like; Ets transcription factor Spi-B; Spi-1/PU.1 related; Spib; SPI-B; Spi-B transcription factor; Spi-B transcription factor (Spi-1/PU.1 related); Transcription factor Spi-B
• Gene Symbols: SPIB
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: SPIB

• Clone: 235D
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: Q01892
• Gene ID (Entrez): 6689
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody