Invitrogen™ SPIN2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5121096

Catalog No. PIPA5121096

Description

Positive test controls include: NCI-H460, MCF7, Mouse liver, Mouse brain, Mouse heart, Mouse stomach, Rat kidney. The target is usually found in the following locations: Nucleus. Immunogen sequence: MKTPNAQEAE GQQTRAAAGR ATGSANMTKK KVSQKKQRGR PSSQPRRNIV GCRISHGWKE GDEPITQWKG TVLDQVPINP SLYLVKYDGI DCVYGLELHR DERVLSLKIL SDRVASSHIS DANLANTIIG KAVEHMFEGE HGSKDEWRGM VLAQAPIMKA WFYITYEKDP VLYMYQLLDD YKEGDLRIMP ESSESPPTER EPGGVVDGLI GKHVEYTKED GSKRIGMVIH QVEAKPSVYF IKFDDDFHIY VYDLVKKS.

Human SPIN2 expressed by 2 IL3-dependent murine myeloid cell lines was moderately antiapoptotic following IL3 removal, but it was unable to block apoptosis induced by the chemotherapy agent doxorubicin. On the contrary, SPIN2-expressing cells were more sensitive to doxorubicin-mediated cell death. Also, SPIN2 did not combat the proapoptotic effects of Fas ligand stimulation in the Jurkat human T-cell line. Deletion of the C-terminal amino acids of SPIN2 diminished its antiapoptotic activity. In the murine myeloid cells, SPIN2 overexpression increased the cell number doubling times and slowed their rate of growth. There was also an increased percentage of cells in G2/M, which was more pronounced following IL3 withdrawal.

Specifications

• Antigen: SPIN2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SPIN2B
• Gene Alias: BC068162; dJ323P24.2; Spin2; SPIN-2; SPIN2_duplicate; SPIN2B; SPIN-2B; Spin2c; SPIN-2C; Spindlin 2B; spindlin family member 2B; spindlin family, member 2; spindlin family, member 2 duplicate; spindlin family, member 2B; spindlin family, member 2C; spindlin-2; spindlin-2B; Spindlin-2C; spindlin-like protein 2; spindlin-like protein 2B; Spindlin-like protein 2C; TDRD26; telomeric SPIN2 copy
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 278240, 474343
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.95 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: Q6NVE3, Q9BPZ2
• Gene Symbols: SPIN2B, Spin2c
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human SPIN2B (NP_001006682.1).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG