Invitrogen™ SPT3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA540315

Catalog No. PIPA540315

Description

Peptide sequence: MRKDKKKLRR LLKYMFIRDY KSKIVKGIDE DDLLEDKLSG SNNANKRQKI Sequence homology: Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 86%; Horse: 100%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 86%; Rat: 93%.

The transcription of many RNA polymerase II-dependent genes requires Spt3, a member of the S. cerevisiae SAGA complex. Transcription from delta sequences, the long terminal repeats that flank yeast Ty elements, requires the yeast SPT3 gene. Spt3 and Spt20 work together to recruit TATA-box binding protein (TBP) to the core promoter allowing TBP to bind to SAGA-dependent promoters. Null mutations in the Spt3 gene cause defects in sporulation, diploid filamentous growth, and haploid invasive growth, indicating that Spt3 has an important role in both mating and development pathways in yeast. At the promoters of some genes including yeast HO, HIS3 and TRP3 genes, Spt3 inhibits binding of TBP, resulting in reduced transcription. This repressive effect of Spt3 can be overcome by another member of the SAGA complex, GCN5, which promotes the formation of a TBP/TFIIA complex by histone acetylation.

Specifications

• Antigen: SPT3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SUPT3H
• Gene Alias: Cdc5-like protein; D9509.12; Positive regulator of Ty transcription; Protein SPT3; SPT3; SPT3 homolog, SAGA and STAGA complex component; SPT3L; SPT3-like protein; suppressor of Ty 3 homolog; suppressor of Ty 3 homolog (S. cerevisiae); SUPT3H; Transcription initiation protein SPT3 homolog; transcriptional regulator SPT3; YDR392W
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 8464
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 2% sucrose and 0.09% sodium azide
• Gene Accession No.: O75486
• Gene Symbols: SUPT3H
• Immunogen: Synthetic peptide directed towards the N-terminal of human SUPT3H (aa 87-136).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov