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Invitrogen™ SPT3 Polyclonal Antibody
Rabbit Polyclonal Antibody
Supplier: Invitrogen™ PA582880
Description
Immunogen sequence: FRDWLDCSSM EIKPNVVAME ILAYLAYETV AQLVDLALLV RQDMVTKAGD PFSHAISATF IQYHNSAEST AACGVEAHSD AIQPCHIREA IRRYSHRIGP LSPFT.
The transcription of many RNA polymerase II-dependent genes requires Spt3, a member of the S. cerevisiae SAGA complex. Transcription from delta sequences, the long terminal repeats that flank yeast Ty elements, requires the yeast SPT3 gene. Spt3 and Spt20 work together to recruit TATA-box binding protein (TBP) to the core promoter allowing TBP to bind to SAGA-dependent promoters. Null mutations in the Spt3 gene cause defects in sporulation, diploid filamentous growth, and haploid invasive growth, indicating that Spt3 has an important role in both mating and development pathways in yeast. At the promoters of some genes including yeast HO, HIS3 and TRP3 genes, Spt3 inhibits binding of TBP, resulting in reduced transcription. This repressive effect of Spt3 can be overcome by another member of the SAGA complex, GCN5, which promotes the formation of a TBP/TFIIA complex by histone acetylation.
Specifications
SPT3 | |
Polyclonal | |
Unconjugated | |
SUPT3H | |
Cdc5-like protein; D9509.12; Positive regulator of Ty transcription; Protein SPT3; SPT3; SPT3 homolog, SAGA and STAGA complex component; SPT3L; SPT3-like protein; suppressor of Ty 3 homolog; suppressor of Ty 3 homolog (S. cerevisiae); SUPT3H; Transcription initiation protein SPT3 homolog; transcriptional regulator SPT3; YDR392W | |
Rabbit | |
Antigen Affinity Chromatography | |
RUO | |
8464 | |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. | |
Liquid |
Immunohistochemistry (Paraffin), Immunocytochemistry | |
0.1 mg/mL | |
PBS with 40% glycerol and 0.02% sodium azide; pH 7.2 | |
O75486 | |
SUPT3H | |
Recombinant protein corresponding to Human SUPT3H. Recombinant protein control fragment (Product #RP-89431). | |
100 μL | |
Primary | |
Human | |
Antibody | |
IgG |
Safety and Handling
WARNING: Cancer - www.P65Warnings.ca.gov
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