Invitrogen™ SPT6 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5120450

Catalog No. PIPA5120450

Description

Positive test controls include: U-87MG, Jurkat, HeLa, Mouse brain, Mouse heart, Mouse liver. The target is usually found in the following locations: Nucleus. Immunogen sequence: IFPTVNGLFR WFKDHYQDPV PGITPSSSSR TRTPASINAT PANINLADLT RAVNALPQNM TSQMFSAIAA VTGQGQNPNA TPAQWASSQY GYGGSGGGSS AYHVFPTPAQ QPVATPLMTP SYSYTTPSQP ITTPQYHQLQ ASTTPQSAQA QPQPSSSSRQ RQQQPKSNSH AAIDWGKMAE QWLQEKEAER RKQKQRLTPR PSPSPMIEST PMSIAGDATP LLDEMDR.

Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A.

Specifications

• Antigen: SPT6
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SUPT6H
• Gene Alias: 5131400N11Rik; AI132449; emb-5; Histone chaperone suppressor of Ty6; hSPT6; Kiaa0162; MGC87943; mKIAA0162; SPT6; SPT6 homolog, histone chaperone; SPT6H; suppressor of Ty 6; suppressor of Ty 6 homolog; suppressor of Ty 6 homolog (S. cerevisiae); Supt6; Supt6h; Tat-cotransactivator 2 protein; tat-CT2 protein; Transcription elongation factor SPT6
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20926, 303281, 6830
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 3.74 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: Q62383, Q7KZ85
• Gene Symbols: SUPT6, SUPT6H
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1500-1726 of human SUPT6H (NP_003161.2).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG