Invitrogen™ SPTBN1 Monoclonal Antibody (4C3)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA3062

Catalog No. MA3062

Description

MA3-062 detects spectrin from mouse, rat and human erythrocytes, brain and muscle cells. This antibody has been shown to specifically detect the two known alternatively spliced forms of spectrin, beta-1 epsilon-1, present in erythrocytes, and beta-1 epsilon-2, present in nerve and striated muscle cells. It does not cross-react with alpha-2 spectrin or either of the fodrin subunits. MA3-062 has been successfully used in Western blot, immunohistochemistry (paraffin) and immunofluorescence procedures. By Western blot, this antibody detects a ~246 kDa protein representing beta-1 spectrin in rat skeletal muscle homogenate. Immunofluorescence staining of beta-1 spectrin in rat skeletal muscle fibers with MA3-062 results in intense staining of the sarcolemma. In immunofluorescence, antigen integrity can be compromised if aldehyde fixatives are left in contact with the protein for extended periods of time. If paraformaldehyde is used as a fixative, exposure should be limited to 5 minutes or less of no more than a 2% solution. The MA3-062 antigen is purified human erythrocyte beta-1 spectrin.

Spectrin (Sp), the most abundant of the erythrocyte membrane skeleton proteins, helps these cells maintain their characteristic biconcave shape while remaining flexible and elastic. Erythrocyte Sp is a heterodimer composed of a 280 kDa alpha subunit and a 246 kDa beta subunit which associate in a side-to-side, antiparallel configuration to form a 100 nm rod-like structure. Sp in other tissues may be composed of distinct but homologous alpha and beta subunits, sometimes referred to as fodrin. A newly introduced nomenclature designates the Sp subunits of the erythrocyte as alpha-1 and beta-1, and the fodrin subunits as alpha-2 and beta-2. Alternatively spliced forms of each are designated as epsilon-1, epsilon-2, etc. (e. g. beta-1 epsilon-1).

Specifications

• Antigen: SPTBN1
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: ascites, PBS with 0.05% sodium azide
• Gene Accession No.: Q01082, Q62261
• Gene Symbols: SPTBN1
• Immunogen: Purified human erythrocyte beta-1 spectrin.
• Regulatory Status: RUO
• Gene ID (Entrez): 20742, 305614, 6711
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 4C3
• Conjugate: Unconjugated
• Gene: SPTBN1
• Gene Alias: 9930031C03Rik; A2a; a--fodrin; AI842465; AL033301; alpha II spectrin; alpha-fodrin; alpha-II spectrin; alpha-spectrin 2; beta fodrin; beta II spectrin-like (short form); beta-fodrin; beta-G spectrin; beta-I spectrin; Beta-II spectrin; beta-spectrin 1; beta-spectrin 2; beta-spectrin 2, non-erythrocytic; beta-spectrin II; beta-spectrin non-erythrocytic 1; betaSpII; brain alpha-spectrin; brain erythroid spectrin (235E); brain spectrin; bSPII-; cytoskeleton protein; D330027P03Rik; EL3; ELF; elf1; elf3; embryonic liver beta-fodrin; embryonic liver fodrin; embryonic liver fodrin beta chain; epididymis luminal protein 102; erythroid spectrin beta; fodrin alpha chain; Fodrin beta chain; Gm1301; HEL102; HS2; HSPTB1; inhibitory protein factor; IPF; ja; jaundiced; membrane cytoskeletal protein; mKIAA4049; mKIAA4219; noerythroid alpha-spectrin 2; non-erythrocytic; nonerythroid alpha-spectrin 2; non-erythroid spectrin beta; short form of beta II spectrin; Sp beta; spectrin alpha chain, brain; spectrin alpha chain, non-erythrocytic 1; spectrin beta 1; spectrin beta 2; spectrin beta chain, brain 1; spectrin beta chain, erythrocyte; spectrin beta chain, erythrocytic; Spectrin beta chain, non-erythrocytic 1; spectrin beta Tandil; spectrin beta, erythrocytic; spectrin beta, non-erythrocytic 1; spectrin G; spectrin R; spectrin, alpha, non-erythrocytic 1; spectrin, beta, erythrocytic; spectrin, beta, non-erythrocytic 1; spectrin, non-erythroid alpha chain; spectrin, non-erythroid beta chain 1; SPH2; Spna2; Spnb1; Spnb-1; Spnb2; Spnb-2; Spta2; Sptan1; SPTB; SPTB1; Sptb2; Sptbn1
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov