Invitrogen™ SPTLC2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA531130

Catalog No. PIPA531130

Description

Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7, mouse liver. Predicted reactivity: Mouse (95%), Rat (95%), Xenopus laevis (80%), Chicken (84%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Serine palmitoyltransferase (SPT), which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPT2 is the long chain base subunit 2 of mammalian serine palmitoyltransferase. SPT2 is catalytically active but needs its related protein SPT1 for its stabilization and anchoring the holoenzyme to the cytosolic face of the endoplasmic reticulum. As in the case with SPT1, mutations in the SPT2 gene can cause hereditary sensory and autonomic neuropathy type I (HSAN-I), resulting from a loss of SPT activity as well as the accumulation of the atypical and neurotoxic sphingoid metabolite 1-deoxy-sphinganine.

Specifications

• Antigen: SPTLC2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SPTLC2
• Gene Alias: AI173915; hLCB2a; HSN1C; KIAA0526; LCB 2; LCB2; LCB2a; long chain base biosynthesis protein 2; Long chain base biosynthesis protein 2a; mKIAA0526; NSAN1C; Pomt2; protein-O-mannosyltransferase 2; serine palmitoyltransferase 2; serine palmitoyltransferase long chain base subunit 2; serine palmitoyltransferase, long chain base subunit 2; serine palmitoyltransferase, subunit II; serine-palmitoyl-CoA transferase 2; SPT 2; SPT2; Sptlc2
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20773, 9517
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.41 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: O15270, P97363
• Gene Symbols: SPTLC2
• Immunogen: Recombinant fragment corresponding to a region within amino acids 50 and 274 of Human Serine palmitoyltransferase 2.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG