MilliporeSigma™ SPTLC3, Rabbit, Unlabeled, Polyclonal,

Catalog No.	Quantity	Conjugate
ABS1640	100 μL	Unconjugated

Catalog No. ABS1640 Supplier MilliporeSigma™ Supplier No. ABS1640

Description

Rabbit Polyclonal Antibody

Serine palmitoyltransferase 3 (EC 2.3.1.50; UniProt Q9NUV7; also known as LCB 3, LCB2b, Long chain base biosynthesis protein 2b, Long chain base biosynthesis protein 3, Serine-palmitoyl-CoA transferase 3, SPT 3) is encoded by the SPTLC3 (also known as C20orf38, SPTLC2L) gene (Gene ID 55304) in human. Serine palmitoyltransferase (SPT) is a pyridoxal 5&prime-phosphate (PLP)-dependent enzyme that catalyses the first step of de novo sphingolipids (SLs) biosynthesis, namely the condensation of L-serine with palmitoyl coenzyme-A to generate 3-ketodehydrosphinganine (KDS) that forms the sphingoid base backbone of all SLs. The core human SPT homoenzyme is a membrane-bound heterodimer composed of an hLCB1 (SPTLC1) subunit together with either an hLCB2a (SPTLC2) or an hLCB2b (SPTLC3) subunit. The hLCB1/hLCB2 heterodimer contains a single active site composed of residues from both subunits. hLCB2a and hLCB2b share 68% sequence identity, with hLCB2b being the isotype that is predominantly expressed in the placenta and prefers shorter chain acyl-CoAs (C12 and C14) to generate short-chain SLs. In addition, two human small subunits (ssSPTa and ssSPTb) have been identified and found to maximize the catalytic activity and to confer the acyl-CoA substrate chain-length specificity of the hLCB1/hLCB2 core enzyme. Mutations in hLCB1 and hLCB2a are found in patients with hereditary sensory and autonomic neuropathy type I (HSAN1), an inherited disorder that affects sensory and autonomic neurons due to neurotoxic deoxysphingolipids formation as a result of substrate promiscuity of the mutated SPT enzyme.

Specifications

• Antigen: SPTLC3
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Rabbit polyclonal antibody serum with 0.05% Sodium Azide.
• Gene: SPTLC3, C20orf38, SPTLC2L
• Gene Accession No.: Q9NUV7
• Host Species: Rabbit
• Immunogen: A mixture of two linear peptides corresponding to sequences near the N- and C-terminal ends of human SPTLC3.
• Purification Method: Unpurified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_060797
• Test Specificity: Target-specificity of this antiserum was verified by negative blotting using pre-immune serum and by target band blocking using immunogen peptides (Hornemann, T., et al. (2006). J. Biol. Chem. 281(49):37275-37281). Expected to react with both spliced isoforms of human Serine palmitoyltransferase 3/SPTLC3 reported by UniProt (Q9NUV7).
• Target Species: Human
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

For Research Use Only. Not for use in diagnostic procedures.