SREBP-1 Monoclonal Antibody (IgG-2A4), Active Motif™

Mouse Monoclonal Antibody

Supplier:  Active Motif 39939

Catalog No. 50-199-2981

Description

Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.

Specifications

• Antigen: SREBP-1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 70mM tris with 0.07mM EDTA, 105mM NaCl, 30% glycerol, 31mM glycine and 0.035% sodium azide; pH 8
• Gene Accession No.: P36956
• Gene Symbols: SREBF1
• Immunogen: This SREBP-1 antibody was raised against a recombinant protein corresponding to amino acids 301-407 of human SREBP-1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: ChIP Assay, Gel Shift, Immunoprecipitation, Western Blot
• Clone: IgG-2A4
• Conjugate: Unconjugated
• Gene: SREBF1
• Gene Alias: ADD1; ADD-1; Adipocyte determination- and differentiation-dependent factor 1; BHLHD1; Class D basic helix-loop-helix protein 1; D630008H06; I79_010155; nt-SREBP1; Processed sterol regulatory element-binding protein 1; SREBF1; SREBP1; SREBP-1; SREBP1a; SREBP-1a; SREBP1c; SREBP-1c; sterol regulatory element binding protein 1; sterol regulatory element binding protein-1; sterol regulatory element binding transcription factor 1; sterol regulatory element binding-protein 1; Sterol regulatory element-binding protein 1; Sterol regulatory element-binding transcription factor 1
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 6720
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid