Invitrogen™ SREBP1 Monoclonal Antibody (2A4)

Catalog No.	Quantity
PIMA516124	100 μL

Catalog No. PIMA516124 Supplier Invitrogen™ Supplier No. MA516124

Description

Mouse Monoclonal Antibody

In Western blot, a band can be seen at 125 kDa (precursor) and additional bands may be seen at 60-70 kDa (cleaved). Suggested positive control: IMR 5 and HeLa cells..

Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.

Specifications

• Antigen: SREBP1
• Applications: Immunohistochemistry (Frozen), Western Blot
• Classification: Monoclonal
• Clone: 2A4
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% sodium azide
• Gene: SREBF1
• Gene Accession No.: P36956, P56720, Q60416, Q9WTN3
• Gene Alias: ADD1; ADD-1; Adipocyte determination- and differentiation-dependent factor 1; bHLHd1; Class D basic helix-loop-helix protein 1; D630008H06; I79_010155; nt-SREBP1; Processed sterol regulatory element-binding protein 1; SREBF1; SREBP1; SREBP-1; SREBP1a; SREBP-1a; SREBP1c; SREBP-1c; sterol regulatory element binding protein 1; sterol regulatory element binding protein-1; sterol regulatory element binding transcription factor 1; sterol regulatory element binding-protein 1; sterol regulatory element-binding protein 1; Sterol regulatory element-binding transcription factor 1
• Gene Symbols: SREBF1
• Host Species: Mouse
• Immunogen: 6 His-tag fusion protein of human SREBP1 corresponding to amino acids 301-407.
• Purification Method: Protein G
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100689018, 20787, 373915, 403475, 6720, 78968
• Target Species: Canine, Chicken, Hamster, Human, Mouse, Monkey, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ