Invitrogen™ SREBP1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5120378

Catalog No. PIPA5120378

Description

Positive test controls include: A-549, MCF7, U-87MG, Mouse liver. The target is usually found in the following locations: COPII-coated vesicle membrane, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Golgi apparatus membrane, Multi-pass membrane protein, Multi-pass membrane protein, Nucleus. Immunogen sequence: SLHTQVQSVV PQQLLTVTAA PTAAPVTTTV TSQIQQVPVL LQPHFIKADS LLLTAMKTDG ATVKAAGLSP LVSGTTVQTG PLPTLVSGGT ILATVPLVVD AEKLPINRLA A.

Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.

Specifications

• Antigen: SREBP1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SREBF1
• Gene Alias: ADD1; ADD-1; Adipocyte determination- and differentiation-dependent factor 1; bHLHd1; Class D basic helix-loop-helix protein 1; D630008H06; I79_010155; nt-SREBP1; Processed sterol regulatory element-binding protein 1; SREBF1; SREBP1; SREBP-1; SREBP1a; SREBP-1a; SREBP1c; SREBP-1c; sterol regulatory element binding protein 1; sterol regulatory element binding protein-1; sterol regulatory element binding transcription factor 1; sterol regulatory element binding-protein 1; sterol regulatory element-binding protein 1; Sterol regulatory element-binding transcription factor 1
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20787, 6720, 78968
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.05% ProClin 300; pH 7.3
• Gene Accession No.: P36956, P56720, Q9WTN3
• Gene Symbols: SREBF1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-310 of human SREBF1 (NP_004167.3).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG