Invitrogen™ SSEA1 Monoclonal Antibody (BRA4F1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA135093

Catalog No. 01-676-933

Description

Bra4F1 reacts with CD15 (220 kDa). CD15 is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. CD15 is further expressed in Reed-Sternberg cells in classic Hodgkin's disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes. It is also expressed on a wide variety of other tumor cells including myeloid leukemia, breast, colorectal, and lung cancer cells. Bra4F1 was clustered at the IVth International Workshop on Leucocyte Differentiation Antigens.

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

Specifications

• Antigen: SSEA1
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: P22083
• Gene Symbols: FUT4
• Immunogen: Erythroid-myeloid leukemia cell line.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgM

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Clone: BRA4F1
• Conjugate: Unconjugated
• Gene: FUT4
• Gene Alias: FUTIV; Lewis X; SSEA-1
• Host Species: Mouse
• Purification Method: Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 2526
• Content And Storage: 4°C
• Form: Liquid