Invitrogen™ SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), Alexa Fluor™ 488, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-168-19	Invitrogen™ 53881342	100 Tests

Description

Description: The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine and human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. Expression of the carbohydrate moiety is also found on mature human granulocytes (on CD15) and some monocytes. SSEA-1 is associated with cell adhesion, migration and differentiation. Applications Reported: This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioMC-480 (MC-480) antibody has been pre-titrated and tested by flow cytometric analysis of F9 cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

Specifications

• Antigen: SSEA1
• Concentration: 5 μL/Test
• Applications: Flow Cytometry
• Conjugate: Alexa Fluor 488
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P22083, Q11127
• Gene ID (Entrez): 14345, 2526
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: eBioMC-480 (MC-480)
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgM
• Target Species: Human, Mouse
• Gene: FUT4
• Gene Alias: FUTIV; Lewis X; SSEA-1
• Gene Symbols: FUT4
• Product Type: Antibody