Invitrogen™ SSEA1 Monoclonal Antibody (MEM-158), APC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA110106

Catalog No. PIMA110106

Description

This antibody reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on the surface of granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. This antibody will not cross-react with porcine, bovine or sheep.

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

Specifications

• Antigen: SSEA1
• Classification: Monoclonal
• Conjugate: APC
• Gene: FUT4
• Gene Alias: FUTIV; Lewis X; SSEA-1
• Host Species: Mouse
• Purification Method: Size-exclusion Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2526
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: MEM-158
• Formulation: TBS with 0.2% BSA and 15mM sodium azide; pH 8
• Gene Accession No.: P22083
• Gene Symbols: FUT4
• Immunogen: Human granulocytes.
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgM