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  6. Invitrogen™ SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), eFluor™ 660, eBioscience™, Invitrogen™ 

Invitrogen™ SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), eFluor™ 660, eBioscience™, Invitrogen™
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Catalog No. 50884382
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50-884-380 25 μg
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Catalog No. 50-884-382 Supplier Invitrogen™ Supplier No. 50884382
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Description

Mouse Monoclonal Antibody

The eBioMC-813-70 MAb reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells. The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys.

SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
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Specifications

Antigen SSEA4
Applications Flow Cytometry, Immunocytochemistry
Classification Monoclonal
Clone eBioMC-813-70 (MC-813-70)
Concentration 0.2 mg/mL
Conjugate eFluor 660
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Alias SSEA-4; stage-specific embryonic antigen 4
Host Species Mouse
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human, Mouse, Canine, Rabbit, Monkey, Chicken
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG3
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Frequently Asked Questions (FAQs)

Is the eFluor 660 fluorochrome compatible with Anti-Cy5/Alexa Fluor 647 beads?

Yes, in-house studies have demonstrated that the eFluor 660 fluorochrome is recognized by Anti-Cy5/Alexa Fluor 647 beads. Side by side studies with Alexa Fluor 647 versus eFluor 660 conjugated antibodies have demonstrated comparable results.
What buffers are compatible with the eFluor Organic fluorochromes and eVolve QDots?

The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.
What is the difference between eFluor Organic Dyes and eVolve Qdots?

The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.
Are the eFluor Organic Dyes photo-labile?

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.
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