Invitrogen™ SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), PerCP-eFluor™ 710, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 46884342

Catalog No. 50-112-3918

Description

Description: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells. The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys. Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an accept...

SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.

Specifications

• Antigen: SSEA4
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: eBioMC-813-70 (MC-813-70)
• Conjugate: PerCP-eFluor 710
• Gene Alias: SSEA-4; stage-specific embryonic antigen 4
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Human, Mouse, Canine, Rabbit, Monkey, Chicken
• Product Type: Antibody
• Isotype: IgG3

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov