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Invitrogen™ SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), PerCP-eFluor™ 710, eBioscience™
Mouse Monoclonal Antibody
$528.00
Specifications
Antigen | SSEA4 |
---|---|
Clone | eBioMC-813-70 (MC-813-70) |
Concentration | 5 μL/Test |
Content And Storage | 4°C, store in dark, DO NOT FREEZE! |
Applications | Flow Cytometry |
Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
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Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
50-112-3918
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Invitrogen™
46884342 |
100 Tests |
Each of 1 for $528.00
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Description
Description: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells. The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys. Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an accept...
SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.Specifications
SSEA4 | |
5 μL/Test | |
Flow Cytometry | |
PerCP-eFluor 710 | |
Mouse | |
RUO | |
PBS with BSA and 0.09% sodium azide; pH 7.2 | |
Primary | |
Affinity chromatography |
eBioMC-813-70 (MC-813-70) | |
4°C, store in dark, DO NOT FREEZE! | |
Monoclonal | |
Liquid | |
IgG3 | |
Human, Mouse, Canine, Rabbit, Monkey, Chicken | |
SSEA-4; stage-specific embryonic antigen 4 | |
Antibody |
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