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Invitrogen™ SSEA5 Monoclonal Antibody (8E11)
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Catalog No. PIMA1144
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100 μg
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Catalog No. Quantity
PIMA1144 100 μg
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Catalog No. PIMA1144 Supplier Invitrogen™ Supplier No. MA1144
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Description

Mouse Monoclonal Antibody

Immunofluorescence and flow cytometric analysis of MA1-144 shows positive staining of SSEA-5 positive pluripotent cells within cultured human embryonal carcinoma NCCIT and NTERA-2 cells. Mouse teratocarcinoma cell line F9 was used as a negative control and showed no staining.

Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide that can be part of glycosphingolipids and glycoproteins. It contains the motif Fuc1-2Gal-beta1-3GlcNAc-beta, which is characteristic of H-1 antigens. SSEA-5 is found in the inner cell mass of the human blastocyst during embryogenesis, on undifferentiated human embryonic stem cells and on the surface of induced pluripotent cells. SSEA-5 was found to proceed the loss of other known stem cell markers and the combination of SSEA-5 with either CD9 and CD90 or CD50 and CD200 was superior in identifying and eliminating rare undifferentiated human ES cells from differentiated cell populations than the use of other established stem cell markers.
TRUSTED_SUSTAINABILITY

Specifications

Antigen SSEA5
Applications Flow Cytometry, Immunocytochemistry
Classification Monoclonal
Clone 8E11
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 1mg/mL BSA and 0.05% sodium azide
Gene Alias SSEA-5
Host Species Mouse
Immunogen Undifferentiated H9 human embryonic stem cells.
Purification Method Protein A
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Liquid
Isotype IgG1 κ
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