Invitrogen™ SSEA5 Monoclonal Antibody (8E11)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA1144

Catalog No. PIMA1144

Description

Immunofluorescence and flow cytometric analysis of MA1-144 shows positive staining of SSEA-5 positive pluripotent cells within cultured human embryonal carcinoma NCCIT and NTERA-2 cells. Mouse teratocarcinoma cell line F9 was used as a negative control and showed no staining.

Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide that can be part of glycosphingolipids and glycoproteins. It contains the motif Fuc1-2Gal-beta1-3GlcNAc-beta, which is characteristic of H-1 antigens. SSEA-5 is found in the inner cell mass of the human blastocyst during embryogenesis, on undifferentiated human embryonic stem cells and on the surface of induced pluripotent cells. SSEA-5 was found to proceed the loss of other known stem cell markers and the combination of SSEA-5 with either CD9 and CD90 or CD50 and CD200 was superior in identifying and eliminating rare undifferentiated human ES cells from differentiated cell populations than the use of other established stem cell markers.

Specifications

• Antigen: SSEA5
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Immunocytochemistry
• Clone: 8E11
• Conjugate: Unconjugated
• Gene Alias: SSEA-5
• Immunogen: Undifferentiated H9 human embryonic stem cells.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C
• Form: Liquid