Invitrogen™ Staphylococcus aureus Cas9 Monoclonal Antibody (6H4), Alexa Fluor™ 647, eBioscience™, Invitrogen™

Catalog No.	Quantity
51-649-982	100 μg
51-649-980	25 μg

Catalog No. 51-649-982 Supplier Invitrogen™ Supplier No. 51649982

Description

Mouse Monoclonal Antibody

Description: This 6H4 monoclonal antibody recognizes Staphylococcus aureus Cas9 endonuclease. Applications Reported: This 6H4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 6H4 antibody has been tested by intracellular staining followed by flow cytometric analysis of Staphylococcus aureus derived Cas9-GFP transfected 293T cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins located at www.thermofisher.com/flowprotocols. This may be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.

Specifications

• Antigen: Staphylococcus aureus Cas9
• Applications: Flow Cytometry, Immunocytochemistry
• Classification: Monoclonal
• Clone: 6H4
• Concentration: 0.2 mg/mL
• Conjugate: Alexa Fluor 647
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: cas9; CRISPR9; CRISPR-Cas9; s aureus
• Host Species: Mouse
• Immunogen: His-tagged recombinant Cas-9 from Staphylococcus aureus (maps to N-terminus).
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Bacteria
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b κ

Safety and Handling

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