Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. Invitrogen™ Staphylococcus aureus Cas9 Monoclonal Antibody (6H4), PerCP-eFluor™ 710, eBioscience™, Invitrogen™ 

Invitrogen™ Staphylococcus aureus Cas9 Monoclonal Antibody (6H4), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
GREENER_CHOICE

Catalog No. 502550816
Encompass_Preferred
Change view
Click to view available options
Quantity:
100 μg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
50-255-0816 100 μg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. 50-255-0816 Supplier Invitrogen™ Supplier No. 46649982
Only null left
Add to Cart
Add to Cart

Description

Mouse Monoclonal Antibody

This 6H4 MAb recognizes Staphylococcus aureus Cas9 endonuclease.

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Staphylococcus aureus Cas9
Applications Flow Cytometry
Classification Monoclonal
Clone 6H4
Concentration 0.2 mg/mL
Conjugate PerCP-eFluor 710
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Alias cas9; CRISPR9; CRISPR-Cas9; s aureus
Host Species Mouse
Immunogen His-tagged recombinant Cas-9 from Staphylococcus aureus (maps to N-terminus).
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Bacteria
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2b κ
Show More Show Less
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.