Stat3 (pY705) Mouse, PE, Clone: 4/P-STAT3, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 612569
Stat proteins function as both cytoplasmic signal transducers and activators of transcription. Stat3 has been reported to be a 92kDa protein that is activated as a DNA binding protein through cytokines, such as IL-6, and growth factors, such as EGF. Stat3 activation occurs via tyrosine phosphorylation at Tyr-705. Tyrosine phosphorylation in response to cytokine stimulation is generally mediated by JAK1. Upon activation, Stat3 dimerizes, translocates to the nucleus, and binds DNA response elements thereby regulating gene expression. It has been reported that Stat3 binds to DNA as a homodimer, but it is also capable of binding as a heterodimer with Stat1. In addition to tyrosine phosphorylation, Stat3 is also phosphorylated at Ser-727 via the MAPK pathway. Stat3 is widely expressed and can bind to the sis-inducible element (SIE) site from the c-fos promoter. This site is similar to the GAS element that is present in IFN-γ induced genes. Thus, phosphorylation of Tyr-705 in Stat3 occurs in response to growth factors and cytokines, and is essential for normal transcription activity. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Flow Cytometry, Intracellular Staining
|Acute-phase response factor, APRF|
|Phosphorylated Human Stat3 (Y705) Peptide|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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