MilliporeSigma™ Stat3b, Mouse, Unlabeled, Clone: 516 G10H9,

Catalog No.	Quantity	Conjugate
MABC1177MI	100 μg	Unconjugated

Catalog No. MABC1177MI Supplier MilliporeSigma™ Supplier No. MABC1177

Description

Mouse Monoclonal Antibody

Signal transducer and activator of transcription 3 (UniProt P40763; also known as Acute-phase response factor, DNA-binding protein APRF) is encoded by the STAT3 (also known as ADMIO, APRF, HIES) gene (Gene ID 6774) in human. STAT proteins (1, 2, 3, 4, 5A, 5B, 6) constitute a family of proteins that mediate cytokine and growth factor receptors-initiated signaling events. Stat3 is activated upon phosphorylation on Y705 by Jak or receptor tyrosine kinases, leading to Stat3 homodimerization, nuclear translocation, and target genes transcription. Constitutively tyrosine-phosphorylated Stat3 is implicated in the initiation and progression of many types of cancers, while Stat3 gene knockout in mice is lethal at the early embryonic stage. Two major alternatively spliced Stat3 isoforms exist, the C-terminal end 55-amino acid sequence (a.a. 716-770) in the canonical alpha isoform (UniProt P40763-1) is truncated and replaced with a 7-amino acid sequence in the 722-amino acid beta isoform (UniProt P40763-3). Tyrosine-phosphorylated Stat3β homodimers are more stable and less susceptible to dephosphorylation, bind DNA more avidly and exhibit markedly prolonged nuclear retention than pStat3α homodimers.

Specifications

• Antigen: Stat3b
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 516 G10H9
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene: STAT3, ADMIO, APRF, HIES
• Gene Accession No.: P40763-3
• Host Species: Mouse
• Immunogen: Linear peptide containing human Stat3b C-terminal end 7 a.a. (CT7).
• Purification Method: Protein G Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Apoptosis & Cancer
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_998827
• Test Specificity: Clone 516 G10H9 detects the C-terminal end 7-a.a. (CT7) sequence present in human/mouse spliced isoform Stat3B (UniProt P40763-3/P42227-2), but not in human/mouse spliced isoform Stat3A (UniProt P40763-1/P42227-1) or human/mouse isoform Del-701 (UniProt P40763-2/P42227-3).
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Form: Purified
• Isotype: IgG1 κ

For Research Use Only. Not for use in diagnostic procedures.