Gibco™ StemPro™ Osteogenesis Differentiation Kit

Catalog No.	Quantity
A1007201	1 Kit

Catalog No. A1007201 Supplier Gibco™ Supplier No. A1007201

Description

StemPro™ Osteogenesis Differentiation Kit

For Complete Differentiation of Human Mesenchymal Stem Cells into Bone Cells

StemPro™ Osteogenesis Differentiation Kit has been developed for the osteogenic differentiation of mesenchymal stem cells (MSCs) in tissue culture vessels. The kit contains all reagents required for inducing MSCs to be committed to the osteogenesis pathway and generate
immature and mature osteocytes. Using StemPro™ Osteogenesis Differentiation Kit in combination with MesenPRO RS™ Medium or StemPro™ MSC SFM provides a standardized culture workflow solution for MSC isolation, expansion and differentiation into mineralized matrix-producing osteocytes.

Specifications

• Cell Type: Stem Cells (Mesenchymal)
• Content And Storage: This kit is shipped in two parts with separate storage requirements. These components are not sold individually:
  • StemPro™ Osteocyte/Chondrocyte Differentiation Basal Medium (100 ml; store in the dark at 2°C to 8°C)
  • StemPro™ Osteogenesis Supplement (10 ml; store in the dark at -5°C to -20°C)
  
  The Basal Medium ships at room temperature, while the Supplement ships on dry ice.
• Culture Environment: CO2
• For Use With (Application): Stem Cell Differentiation
• Product Type: Osteogenesis Differentiation Kit
• Endotoxin Level: ≤50 EU/mL, ≤ 50 EU/ml
• Format: Liquid
• Glucose: Low Glucose
• Glucose Concentration: 1000 mg/L
• Glutamine: GlutaMAX™-I
• HEPES Buffer: No HEPES
• Inorganic Salts: Sodium Phosphate
• Phenol Red Indicator: No Phenol Red
• Product Line: Gibco, StemPro
• Purity or Quality Grade: Research Grade
• Quantity: 1 Kit
• Research Category: Differentiation
• Serum Level: Standard Serum
• Sodium Bicarbonate Buffer: Sodium Bicarbonate
• Sodium Pyruvate Additive: Sodium Pyruvate
• Species: Human
• Supplements: Osteogenesis Supplements

Frequently Asked Questions (FAQs)

What kinds of growth factors are used in mesenchymal stem cell (MSC) research?

Growth factors used for MSC maintenance and self-renewal: LIF, FGF-basic, EGF, HGF, PDGF, and Wnt3.

Growth factors or cytokines for MSC differentiation: BMP-2and TGF-β1.

(Arthritis Res Ther 9:204 (2007)

View our Gibco™ MSC research products.

How can I culture mesenchymal stem cells (MSCs) in vitro?

MSCs may be cultured in either serum-containing medium or serum-free medium. The standard culturing conditions are DMEM, low glucose with 10% FBS. MSCs can also be grown in reduced-serum (2%) Gibco MesenPRO RS Medium (Cat. No. 12746-012), or in serum-free Gibco StemPro MSC SFM XenoFree Medium (Cat. No. A10675) and Gibco StemPro MSC SFM (Cat. No. A10332). In general, MSCs grow better under hypoxic conditions (2% O2).

How do you characterize human mesenchymal stem cells (MSCs)?

Human MSCs can be identified by flow cytometry, typically displaying CD73+, CD90+, CD105+, CD11b-, and CD45- marker characteristics (Blood 109:4245 (2007)). Human MSC expresses Oct4, Sox2, and Rex-1; these may be verified using RT-PCR (Arthritis Res Ther 9:204 (2007)).

What are mesenchymal stem cells?

Mesenchymal stem cells (MSCs) are multipotent cells isolated primarily from bone marrow or fat tissues that exhibit the ability to differentiate into bone, cartilage, and fat cells. Under normal cell culture conditions, MSCs isolated from bone marrow are spindle shaped with the unique ability to adhere to uncoated plastic culture dishes (Arthritis Res Ther 9:204 (2007)).

For Research Use Only. Not for use in diagnostic procedures.