Invitrogen™ STK29 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA520358

Catalog No. PIPA520358

Description

A suggested positive control is human brain tissue lysate. PA5-20358 can be used with blocking peptide PEP-0478.

BRSK2 is a brain-selective serine/threonine kinase 2 that is mainly expressed in the brain, with weaker expression in testis and pancreas. BRSK2 expressed in insect cells specifically phosphorylates WEE1A, CDC25C and CDC25B in an in vitro assay. DNA damage induced by ultraviolet (UV) irradiation or methyl methane sulfonate, but not by ionizing radiation, enhanced endogenous BRSK2 kinase activity in a caffeine-sensitive manner and caused translocation of BRSK2 from the cytoplasm to the nucleus. Overexpression of BRSK2 in HeLa cells results in increased phosphorylation of CDC25C. DNA damage induced by ultraviolet (UV) irradiation or methyl methane sulfonate, but not by ionizing radiation, enhances endogenous BRSK2 kinase activity in a caffeine-sensitive manner and causes translocation of BRSK2 from the cytoplasm to the nucleus. Overexpression of BRSK2 induces G2/M arrest in HeLa cells. Small interfering RNA against BRSK2 partly abrogates UV-induced G2/M arrest. BRSK2 may act as a checkpoint kinase upon DNA damage induced by UV irradiation or methyl methane sulfonate.

Specifications

• Antigen: STK29
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Brsk2
• Gene Alias: 4833424K13Rik; BR serine/threonine kinase 2; BR serine/threonine-protein kinase 2; brain serine/threonine kinase 2; brain-selective kinase 2; Brain-specific serine/threonine-protein kinase 2; BRSK2; C11orf7; HUSSY-12; Kiaa4256; PEN11B; protein kinase SAD1B; SAD1; SAD1B; Sada; SAD-A; serine/threonine-protein kinase 29; Serine/threonine-protein kinase BRSK2; serine/threonine-protein kinase SAD-A; STK29; testicular tissue protein Li 30
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 75770, 9024
• Content And Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
• Form: Liquid

• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: Q69Z98, Q8IWQ3
• Gene Symbols: Brsk2
• Immunogen: A 23 amino acid peptide from near the carboxy terminus of human BRSK2.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov