Invitrogen™ Strep Tag II Monoclonal Antibody (A5D11)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA542540

Catalog No. PIMA542540

Description

Positive Control: Strep-Tag II protein transfected 293F cells lysate, Non-transfected 293F cells lysate.

The Strep-tag II is an eight-residue minimal peptide sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) that exhibits intrinsic affinity toward streptavidin and can be fused to recombinant proteins in various fashions. The original Strep-tag was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.

Specifications

• Antigen: Strep Tag II
• Classification: Monoclonal
• Concentration: 2 mg/mL
• Formulation: TBS with 0.2% BSA, 50% glycerol and 0.05% sodium azide; pH 7.4
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Tag
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Clone: A5D11
• Conjugate: Unconjugated
• Gene Alias: Streptavidin; Streptomyces
• Immunogen: Synthetic peptide corresponding to Strep-tag II.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid
• Target Molecule: Tag