Streptavidin Polyclonal Antibody, FITC, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 200402095

Catalog No. 50-199-0939

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-Streptavidin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and Streptavidin. No reaction was observed against Avidin.

Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of ∼ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

Specifications

• Antigen: Streptavidin
• Classification: Polyclonal
• Conjugate: FITC
• Host Species: Rabbit
• Quantity: 100 μg
• Primary or Secondary: Primary
• Product Type: Antibody
• Target Molecule: Tag

• Applications: ELISA, Immunofluorescence, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
• Immunogen: Streptavidin (Streptomyces avidinii)
• Regulatory Status: RUO
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized