Invitrogen™ STT3B Polyclonal Antibody

Catalog No.	Quantity
PIPA5106380	100 μL

Catalog No. PIPA5106380 Supplier Invitrogen™ Supplier No. PA5106380

Description

Rabbit Polyclonal Antibody

Antibody detects endogenous levels of total STT3B.

Catalytic subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. This subunit contains the active site and the acceptor peptide and donor lipid-linked oligosaccharide (LLO) binding pockets. STT3B is present in a small subset of OST complexes and mediates both cotranslational and post-translational N-glycosylation of target proteins: STT3B-containing complexes are required for efficient post-translational glycosylation and while they are less competent than STT3A-containing complexes for cotranslational glycosylation, they have the ability to mediate glycosylation of some nascent sites that are not accessible for STT3A. STT3B-containing complexes also act post-translationally and mediate modification of skipped glycosylation sites in unfolded proteins. Plays a role in ER-associated degradation (ERAD) pathway that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins by mediating N-glycosylation of unfolded proteins, which are then recognized by the ERAD pathway and targeted for degradation. Mediates glycosylation of the disease variant AMYL-TTR 'Asp-38' of TTR at 'Asn-118', leading to its degradation.

Specifications

• Antigen: STT3B
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene: STT3B
• Gene Accession No.: Q3TDQ1, Q8TCJ2
• Gene Alias: 1300006C19Rik; B6dom1 antigen; CDG1X; dolichyl-diphosphooligosaccharide protein glycotransferase; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B; homolog of yeast STT3; oligosaccharyl transferase subunit STT3B; RGD1311563; Simp; Source of immunodominant MHC-associated peptides; source of immunodominant MHC-associated peptides homolog; STT3, subunit of the oligosaccharyltransferase complex, homolog B; STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae); STT3B; STT3-B; STT3B, catalytic subunit of the oligosaccharyltransferase complex; STT3B, subunit of the oligosaccharyltransferase complex (catalytic)
• Gene Symbols: STT3B
• Host Species: Rabbit
• Immunogen: A synthesized peptide derived from human STT3B(Accession Q8TCJ2), corresponding to amino acid residues C715-W765.
• Purification Method: Affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 201595, 363160, 68292
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Safety and Handling

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