Invitrogen™ STUB1 Monoclonal Antibody (13I8)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA549189

Catalog No. PIMA549189

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human HEK293 whole cell, monkey COS-7 whole cell, human CACO-2 whole cellhuman THP-1 whole cell, human PC-3 whole cell, human U87 whole cell, rat heart tissue, rat brain tissue, mouse brain tissue, mouse HEPA1-6 whole cell. IHC: human colon cancer tissue, human lung cancer tissue, human mammary cancer tissue, mouse small intestine tissue, rat small intestine tissue. Flow: A549 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

A recently identified protein, termed carboxyl terminus of hsc70-interacting protein (CHIP), has been shown to interact both with the constitutive form of hsc70 and the stress inducible form, hsp70. This novel 35 kDa cytoplasmic protein has been shown to be highly expressed in striated muscle in vivo. Additional studies have shown that this protein is expressed over a broad range of cultured tissues. Through immunoprecipitation experiments, CHIP has been shown to directly bind to the carboxyl terminus of hsc70 and hsp70 where it decreases ATPase activity and reduces overall chaperone efficiency. CHIP has also been identified as an important protein in the ubiquitin-proteasome system. CHIP contains a U-box domain and acts as an E3 ubiquitin-ligase in conjunction with hsc70 and hsp90.

Specifications

• Antigen: STUB1
• Classification: Monoclonal
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and 0.05mg sodium azide
• Gene Accession No.: Q9UNE7, Q9WUD1
• Gene Symbols: STUB1
• Immunogen: E.coli-derived human STUB1 recombinant protein (Position: R51-Y303).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Monkey
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Clone: 13I8
• Conjugate: Unconjugated
• Gene: STUB1
• Gene Alias: 0610033N24Rik; 2210017D18Rik; 2310040B03Rik; Antigen NY-CO-7; AW046544; Carboxy terminus of Hsp70-interacting protein; CHIP; CLL-associated antigen KW-8; E3 ubiquitin-protein ligase CHIP; heat shock protein A binding protein 2 (c-terminal); HSPABP2; LA16c-313D11.6; NY-CO-7; PP1131; RING-type E3 ubiquitin transferase CHIP; SCAR16; SDCCAG7; serologically defined colon cancer antigen 7; STIP1 homology and U box-containing protein 1; STIP1 homology and U-box containing protein 1; STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase; Stub1; UBOX1
• Host Species: Mouse
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10273, 287155, 56424
• Content And Storage: -20°C
• Form: Lyophilized