Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

SUMO-2/3, Mouse anti-Human, Clone: 8A2, Millipore Sigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABS203925UG

Catalog No. MABS203925


Only null left
Add to Cart

Description

Description

Anti-SUMO-2/3, clone 8A2, Cat. No. MABS2039, is a mouse monoclonal antibody that detects SUMO-2 and SUMO-3 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Small ubiquitin-related modifier 2 (UniProt P61956; also known as HSMT3, Sentrin-2, SMT3 homolog 2, Smt3B, SUMO-2, Ubiquitin-like protein SMT3B) and 3 (UniProt P55854; also known as SMT3 homolog 1, Smt3A, SUMO-3, Ubiquitin-like protein SMT3A) are encoded by the SUMO2 (also known as SMT3B, SMT3H2; Gene ID 6613) and SUMO3 (also known as SMT3A, SMT3H1; Gene ID 6612) genes in human. SUMOylation, protein post-translation modification by small ubiquitin-like modifier (SUMO), is a signaling event in many cellular processes. SUMO proteins are translated as immature precursors and subsequently converted to their mature forms through the activity of sentrin/SUMO-specific proteases (SENPs). SUMOylation is a reversible process. SUMO E1 activating enzyme, E2 conjugating enzyme, and E3 ligase mediate SUMOylation of substrate proteins, while SENPs are responsible for the de-SUMOylation. SUMOylation usually occurs at lysine residues in the consensus KxD/E motif, although not all such lysines become SUMOylated and SUMOylation can also occur on lysine residues outside of this motif. SUMO2 and 3 share 97% identity at the amino acid level, while SUMO1 shares only about 50% sequence homology with SUMO-2 and 3. In addition to difference in their target substrates, SUMO2/3 can be SUMOylated and form chains, whereas SUMO1 cannot and may serve as chain terminator. SUMO-2 can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Its covalent attachment to its substrate via an isopeptide bond requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Although SUMO-2 functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system, however, unlike ubiquitin which targets proteins for degradation, SUMO-2 is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus (aa 94-95; propeptide) have been cleaved off.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

SUMO-2/3
Monoclonal
Unconjugated
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
SUMO2;SMT3B
GST-tagged full length recombinant human SUMO-2 protein.
25 μg
Signaling
Clone 8A2 detects SUMO-2/3 in human cells.
Stable for 1 year at 2-8°C from date of receipt.
IgG2b κ
Immunocytochemistry, Immunoprecipitation, Western Blot
8A2
Western Blotting Analysis: A representative lot detected SUMO-2/3 in Western Blotting applications (Zhang, X.D., et. al. (2008). Mol Cell. 29(6):729-41; Zhu, S., et. al. (2009). Mol Cell. 33(5):570-80).Immunoprecipitation Analysis: A representative lot detected SUMO-2/3 in Immunoprecipitation applications (Zhu, S., et. al. (2009). Mol Cell. 33(5):570-80).Western Blotting Analysis: A 1:500 dilution from a representative lot detected SUMO-2/3 in HeLa cell lysate (Courtesy of Christine Lee, Matunis Lab, John Hopkins University, Baltimore, Maryland USA).Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected SUMO-2/3 in HeLa cells (Courtesy of Christine Lee, Matunis Lab, John Hopkins University, Baltimore, Maryland USA).Immunocytochemistry Analysis: A representative lot detected SUMO-2/3 in Immunocytochemistry applications (Zhang, X.D., et. al. (2008). Mol Cell. 29(6):729-41; Rao, H.B., et. al. (2017) Science. 355(6323):403-407).Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected SUMO-2/3 in HeLa cells.
Small ubiquitin-related modifier 2;HSMT3;SMT3 homolog 2;SUMO-3;Zentrin-2;Ubiquitin-like protein SMT3B;Smt3B
Mouse
Protein G purified
RUO
Primary
Human
Purified
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Product Certifications
Promotions

Promotions

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.