MilliporeSigma™ SUMO2/3, Rat, Unlabeled, Clone: 3H12,

Catalog No.	Quantity	Conjugate
MABS1139MI	100 μL	Unconjugated

Catalog No. MABS1139MI Supplier MilliporeSigma™ Supplier No. MABS1139

Description

Rat Monoclonal Antibody

Small ubiquitin-related modifier 2 (UniProt P61956; a.k.a. HSMT3, Sentrin-2, SMT3 homolog 2, Smt3B, SUMO-2, Ubiquitin-like protein SMT3B) and 3 (UniProt P55854; a.k.a. SMT3 homolog 1, Smt3A, SUMO-3, Ubiquitin-like protein SMT3A) are encoded by the SUMO2 (a.k.a. SMT3B, SMT3H2; Gene ID 6613) and SUMO3 (a.k.a. SMT3A, SMT3H1; Gene ID 6612) genes in human. SUMOylation, protein posttranslation modification by small ubiquitin-like modifier (SUMO), is a signaling event in many cellular processes. SUMO proteins are translated as immature precursors and subsequently converted to their mature forms through the activity of sentrin/SUMO-specific proteases (SENPs). As is the case with ubiquitination, SUMOylation is a reversible process. SUMO E1 activating enzyme, E2 conjugating enzyme, and E3 ligase mediate SUMOylation of substrate proteins, while SENPs are responsible for the deSUMOylation. SUMOylation usually occurs at lysine residues in the consensus KxD/E motif, although not all such lysines become SUMOylated and SUMOylation can also occur on lysine residues outside of this motif. SUMO2 and 3 share 97% identity at the amino acid level, while SUMO1 shares approximately 50% and SUMO4 shares about 87% identity with SUMO2/3. SUMO4 is the only SUMO member whose ctivation and conjugation has not been demonstrated. In addition to difference in their target substrates, SUMO2/3 can be SUMOylated and form chains, whereas SUMO1 cannot and may serve as chain terminator.

Specifications

• Antigen: SUMO2/3
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 3H12
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Rat IgG2a hybridoma culture supernatant with 0.05% Sodium Azide.
• Gene: SUMO2, SMT3B, SMT3H2, SUMO3, SMT3A, SMT3H1
• Gene Accession No.: P61956;P55854
• Host Species: Rat
• Immunogen: GST-tagged recombinant full-length human SUMO3 (Uchimura, Y., et al. (2006). J. Biol. Chem. 281(32):23180-23190).
• Purification Method: Unpurified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_008868;NP_008867
• Test Specificity: Clone 3H12 detects SUMO2/3 and SUMO2/3-modified (SUMOylated) proteins. Clone 3H12 reacts with SUMO2/3, but not SUMO1 (Uchimura, Y., et al. (2006). J. Biol. Chem. 281(32):23180-23190).
• Target Species: Human
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Isotype: IgG2a κ

For Research Use Only. Not for use in diagnostic procedures.