Invitrogen™ SuperScript™ IV RT-LAMP Master Mix

Description

Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions.

Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions. With this convenient master mix formulation, you can perform isothermal amplification from both RNA or DNA targets in a single tube format. 

Benefits of SuperScript IV RT-LAMP Master Mix
• RNA and DNA target detection using Bst DNA polymerase for isothermal amplification in as little as five minutes
• Robust and specific cDNA synthesis using SuperScript IV Reverse Transcriptase
• Enhanced specificity and sensitivity with buffer formulation and RNaseOUT recombinant ribonuclease inhibitor
• Compatible with real-time and endpoint detection—includes SYTO 9 dye for real-time detection
• Flexible design provides several options for evaluating results, including real-time and endpoint detection methods

SuperScript IV RT-LAMP Master Mix includes an evolved Bst DNA polymerase mutant with a fast reaction speed and increased sensitivity and tolerance to inhibitors, as well as SuperScript IV Reverse Transcriptase with increased processivity, thermostability, and inhibitor resistance. To prevent RNA degradation by carry-over RNases, the master mix includes the potent, noncompetitive RNaseOUT Recombinant Ribonuclease Inhibitor. Our proprietary reaction buffer composition is optimized to increase specificity by delaying no-template control (NTC) amplification time.

Specifications

• Product Type: Master Mix
• For Use With (Application): LAMP, RT-LAMP
• Polymerase: Bst DNA Polymerase
• Quantity: 100 reactions, 1 x 1.25 mL
• Content And Storage:

  • 2X SuperScript IV RT-LAMP Master Mix (1.25 mL)
  • SYTO Green Fluorescent Nucleic Acid Stain (30 μL)
  
  Store at –20°C and protect from light.
  
  

• No. of Reactions: 100 Reactions
• Optimal Reaction Temperature: 65°C
• Reverse Transcriptase: SuperScript IV
• Shipping Condition: Dry Ice
• Detection Method: Real-time and end-point detection
• Includes Label or Dye: SYTO 9 Green Fluorescent Nucleic Acid Stain
• Concentration: 2X
• Reaction Time: 5 to 15 minutes