Thermo Scientific™ SureQuant™ Streptavidin IP-MS Sample Preparation Kit, 20 Reactions PROMO

Description

Thermo Scientific SureQuant IP-MS Sample Preparation Kits provide an optimized solution for the immunoprecipitation and MS peptide preparation of samples upstream of LC-MS/MS analysis.

• Complete—kits include reagents for successful immunoprecipitation and MS peptide preparation for target proteins for SRM/PRM analysis
• Multiplex—IP-MS kits have been designed for multiplex IP using both Protein A/G and Streptavidin magnetic beads
• Flexible—universal reagents enable individualized selection of targets for IP to be used alone or combined with SureQuant peptide quantitation panels
• Optimized—simplified and condensed procedure to prepare samples for LC-MS analysis
• Low background—binding, wash, and elution buffers optimized to remove contaminants and minimize enrichment of background proteins

The SureQuant Protein A/G and Streptavidin IP-MS Sample Preparation kits have been designed to support targeted quantitation using SureQuant or SRM/PRM analysis. The kits contain high-quality Pierce Protein A/G or Streptavidin magnetic beads together with reagents that have been optimized for MS compatibility. The streamlined procedure enables digestion immediately after the immunoprecipitation elution step, facilitating MS sample preparation in 4 hours for same-day LC-MS/MS analysis.

Applications:

• Enrichment of low abundance targets to improve MS sensitivity
• Identification of target proteins that are not detectable by western blotting
• Quantitation of target proteins by LC-MS analysis
• Screening of antibodies for IP-MS
• Enrichment of target proteins upstream of targeted analysis (SRM)

Specifications

• Kit Contents: Trypsin Protease, 2 x 20 μg, Trypsin Storage Solution, 250 μL, Streptavidin Magnetic Beads, 1 mL, IP-MS Cell Lysis Buffer, 100 mL, IP-MS Wash Buffer A, 75 mL, IP-MS Wash Buffer B, 40 mL, IP Elution and MS Sample Prep Buffer, 10 mL, Alkylation Solution, 1 mL/vial, Low Protein-Binding Collection Tubes, 50 x 1.5 mL, Bond-Breaker™ TCEP Solution, Neutral pH, 0.5 mL, 10% Pierce™ Trifluoroacetic Acid (TFA), Sequencing Grade, 0.5 mL
• For Use With (Equipment): Mass Spectrometer
• Product Line: SureQuant™
• Content And Storage: Store trypsin at 4°C. Store trypsin storage solution at -20°C.
• Starting Material: Cells, Tissue
• Detection Method: Mass Spectrometry
• Workflow Step: Sample prep enrichment
• Quantity: 20 Reactions
• For Use With (Application): MS-compatible immunoprecipitation