Fisherbrand™ SYBR™ Green qPCR Master Mix

Description

Fisherbrand™ SYBR™ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.

Features of Fisherbrand SYBR Green qPCR Master Mix include:

• Broad instrument and workflow compatibility enabling Fisherbrand SYBR Green qPCR Master Mix to be used with a wide variety of real-time PCR instruments and reverse transcriptase (RT) enzymes
• Highly sensitive and specific low-level detection of your target of interest
• Wide linear dynamic range—precise measurement across six logs of dynamic range, enabling accurate detection with both low- and high -concentration samples¹

Broad instrument compatibility

Fisherbrand SYBR Green qPCR Master Mix can be used in either standard or fast cycling mode and is compatible with a wide variety of real-time PCR instruments. 

Broad workflow compatibility

Fisherbrand SYBR Green qPCR Master Mix is compatible with a variety of RT enzymes and provides flexibility for varying primer melting temperatures (Tm), between 55°C and 65°C.

Highly sensitive and specific

Fisherbrand SYBR Green qPCR Master Mix enables low-level detection and contains an antibody-mediated hot-start mechanism to mitigate non-specific early polymerase activity. See figure for a performance comparison vs. other leading master mixes.

Wide dynamic range

Fisherbrand SYBR Green qPCR Master Mix can yield up to 6-log dynamic range for cDNA (see figure) and shows equivalent or better dynamic range in the detection of various targets compared to other master mixes.

Other features include:

• Stability of pre-assembled reactions for up to 72 hours in dark room at room temperature²
• Reduce carryover contamination—uracil-DNA glycosylase (UDG) and dUTP included in mix to help ensure leftover DNA residue is not re-amplified in subsequent experiments

Footnotes
1. See user guide for specific sample concentration recommendations.
2. Pre-PCR stability is influenced not only by the master mix, but also by the target being analyzed. For maximum confidence, researchers should confirm stability profiles of their specific targets.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

• Concentration: 2X
• Content And Storage: SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
• Detection Method: SYBR
• Form: Frozen
• GC-Rich PCR Performance: High
• PCR Method: qPCR, 2-step RT-qPCR, RT-qPCR
• Reaction Speed: Fast or Standard
• Label or Dye: SYBR Green, FAM
• Product Type: Master Mix
• Shipping Condition: Dry Ice
• For Use With (Application): Gene Expression, DNA Quantitation, Microbial Detection, Real Time PCR (qPCR)
• No. of Reactions: 100 x 20 μL Reactions
• Volume: 1 mL