Invitrogen™ Synaptophysin Monoclonal Antibody (SP11)

Rabbit Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
MA139558	Invitrogen™ MA139558	1 mL
PIMA516402	Invitrogen™ MA516402	500 μL

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is pancreas or neuroendocrine tumor.

Synaptophysin is a calcium-binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis. It is also involved in the regulation of short-term and long-term synaptic plasticity. Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors. Mutations in the gene can result in mental retardation, X-linked 96.

Specifications

• Antigen: Synaptophysin
• Concentration: Conc. Not Determined
• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Rabbit
• Regulatory Status: RUO
• Formulation: Tissue culture supernatant, TBS with 1% BSA and 0.1% sodium azide; pH 7.5
• Gene Accession No.: P07825, P08247, Q62277
• Gene ID (Entrez): 20977, 24804, 6855
• Immunogen: Synthetic peptide corresponding to C-terminus of human synaptophysin.
• Product Type: Antibody

• Clone: SP11
• Content And Storage: -20°C or -80°C if preferred
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG
• Target Species: Human, Mouse, Rat
• Gene: Syp
• Gene Alias: A230093K24Rik; AI848995; BM89 antigen; I79_012252; Major synaptic vesicle protein p38; MRX96; MRXSYP; p38; Syn; Synaptophysin; Syp; Syp I; Syp1
• Gene Symbols: Syp
• Primary or Secondary: Primary