Invitrogen™ T-Lymphocytes Monoclonal Antibody (KEN-5), FITC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA180464

Catalog No. MA180464

Description

This clone recognizes rabbit (lapine) T-cells, including >90% thymocytes and approximately 40-45% mesenteric lymph node cells. It immunoprecipitates a 67 kDa protein from rabbit thymocytes. This antibody does not bind to rabbit CD5 transfectants and shows very limited binding to rabbit B-cells, which in adult rabbits have been demonstrated to express CD5. This clone may recognize an epitope that is dependant on glycosylation to maintain its structural configuration, which may explain why the antibody does not recognize recombinant rabbit CD5 produced in insect cells (which contain different glycans from those found in mammalian cells). For flow cytometry, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL.

T Lymphocytes are a subtype of white blood cell that is central to cell-mediated immunity. T Lymphocytes mature in the thymus from thymocytes. The majority of human T cells rearrange their alpha and beta chains on the cell receptor and are termed alpha beta T cells (alpha/beta T cells) and are part of the adaptive immune system.

Specifications

• Antigen: T-Lymphocytes
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with 1% BSA and 0.09% sodium azide; pH 7.4
• Immunogen: Rabbit thymocytes.
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: KEN-5
• Conjugate: FITC
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Rabbit
• Product Type: Antibody
• Isotype: IgG1