TACE Human Recombinant anti-Human, Clone: D1(A12), MilliporeSigma™

Human Recombinant Monoclonal Antibody

Supplier:  MilliporeSigma MABT884

Catalog No. MABT884MI

Description

Detect TACE/ADAM17 using this human monoclonal Anti-TACE, clone D1(A12) validated for use in Flow Cytometry and Activity/Function Inhibition.

Specifications

• Antigen: TACE
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Gene Symbols: ADAM17,CSVP,TACE
• Immunogen: See Specificity section.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Cat. No. MABT884 is a recombinant human IgG1 that contains a heavy chain variable (VH) region with affinity towards human TACE noncatalytic Dis-Cys domain and a light chain variable (VL) region that binds human TACE catalytic domain (Tape, C.J., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(14):5578-5583). The VH region of D1(A12) is derived from the scFv named D1 isolated from a phage library generated using pooled lymphocytes from 43 donors (Schofield, D.J., et al. (2007). Genome Biol. 8(11):R254). The D1 VH cDNA was subsequently cloned into a naïve human VL phage-display library for identification and isolation of the hybrid D1(A12) scFv with affinity toward both Dis-Cys domain and the catalytic domain. The D1 VH and A12 VL cDNAs were then cloned into a pBudCE4.1 human IgG1 expression vector for generating the recombinant antibody in HEK293 cells (Tape, C.J., et al. (2011). Proc. Natl. Acad. Sci. USA. 108(14):5578-5583).
• Content And Storage: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Isotype: IgG1 κ

• Applications: Inhibition Assays, Flow Cytometry
• Clone: D1(A12)
• Conjugate: Unconjugated
• Host Species: Human
• Quantity: 100 μg
• Research Discipline: Apoptosis and Cancer
• Gene ID (Entrez): NP_003174
• Target Species: Human
• Form: Purified