TAG-72 Antibody (B72.3), Alexa Fluor™ 700, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP233180AF700

Catalog No. NBP233180Z

Description

TAG-72 Monoclonal specifically detects TAG-72 in Human, Rat, Bovine, Canine, Hamster samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: TAG-72
• Classification: Monoclonal
• Conjugate: Alexa Fluor 700
• Gene Alias: CA 72-4, Protein TAG-72, tag 72, tag-72 Protein TAG-72
• Immunogen: A membrane enriched fraction of a human breast carcinoma liver metastasis was used as the immunogen for the TAG-72 (B72.3) antibody.
• Quantity: 0.1 mL
• Primary or Secondary: Primary
• Test Specificity: Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This monoclonal antibody defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
• Content And Storage: Store at 4C in the dark.
• Isotype: IgG1 κ

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: B72.3
• Dilution: Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1-5 μg/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Gene ID (Entrez): 182875
• Target Species: Human, Rat, Bovine, Canine, Hamster
• Form: Purified

For Research Use Only