Invitrogen™ TAP Tag Monoclonal Antibody (22F12-1E3)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA1108

Catalog No. PIMA1108

Description

MA1-108 detects TAP in Western blot applications. The MA1-108 immunogen is a KLH-conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage. TAP adds about 20 kDa to the size of the protein.

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the TAP tag (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.

Specifications

• Antigen: TAP Tag
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Tag
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunoprecipitation, Western Blot
• Clone: 22F12-1E3
• Conjugate: Unconjugated
• Gene Alias: Tobacco Etch Virus
• Immunogen: KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid
• Target Molecule: Tag