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Description
One unit incorporates 10nmol of total nucleotides into acid-insoluble material in 30 min. at 70°C in a total volume of 50μL.
- Purity: Free from detectable nonspecific nuclease and Taq I restriction endonuclease activities
- Concentration: 5000 units/mL
- Functional testing: Tested in PCR
- Storgae Buffer: 50mM Tris-HCl (pH 7.5), 5mM DTT, 0.1mM EDTA, 50% glycerol, stabilizers
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Specifications
Specifications
| Concentration | 5000 U/mL |
| Content And Storage | 100mM Tris HCl, pH 9, 15mM MgCl2 and 500mM KCl. Store at -20°C |
| pH | 7.5 |
| Purity or Quality Grade | Free from detectable nonspecific nuclease and Taq I restriction endonuclease activities. |
| Quantity | 250U |
| For Use With (Application) | Standard PCR |
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