Applied Biosystems™ TaqMan™ Advanced miRNA Human Endogenous Controls 96-well Plate, standard

The Applied Biosystems TaqMan Advanced Human Endogenous Controls 96-Well Plate, Standard, provides the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well standard plate format.

Supplier:  Applied Biosystems™ A34642

Catalog No. A34642

Description

This product contains 1 plate than can be used to evaluate 30 potential endogenous control miRNAs for up to 3 samples. Two exogenous (non-human) controls are included for normalization of sample input.

Features of the TaqMan Advanced miRNA Human Endogenous Controls 96-Well Plate include:
• Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
• Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
• Updated content—assay content updated for mirBase v.21
• Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
• Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:
Number of plates: 1
Number of unique miRNAs (not including exogenous controls): 30
Exogenous controls: cel-miR-39-3p, ath-miR159a
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.

Specifications

• Content And Storage: Store at room temperature.
• Format: 96-Well Plate
• PCR Method: 2-step RT-qPCR
• For Use With (Equipment): 7500 System, 7900 HT System, QuantStudio™ 12 k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, ViiA™ 7 System, StepOnePlus™ System
• Product Type: Endogenous Control 96-Well Plate
• Shipping Condition: Room Temperature
• Sample Type: Liquid Samples (e.g. Serum), Tissue, Cells, FFPE & Fixed Samples, Blood

• Detection Method: Primer-probe
• GC-Rich PCR Performance: High
• Reaction Speed: Standard
• Product Line: TaqMan™
• Quantity: 1 plate
• For Use With (Application): Microarray Analysis
• Volume: 2 μL

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.