Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents

TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.

Supplier:  Applied Biosystems™ 4308323

Catalog No. 43-083-23

Description

• Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe
• The IPC can be spiked into samples to distinguish true target negatives from PCR inhibition
• This kit allows users to amplify a low-copy target DNA in the same tube with the IPC
• Although the target and IPC DNAs may differ in initial copy number, the concentration of the IPC primers in the PCR reaction is limiting so that the amplification efficiency of the target reaction is not compromised
• Pre-designed primers and TaqMan™ probe eliminate assay design
• Rapid assay development guidelines can minimize optimization time Separate components provide flexibility in assay set-up
• Reagents may be stored at 2°C to 4°C if they are to be consumed within one month
• Otherwise, store at -20°C to -25°C

Note: This is not a quantitative kit and should not be used for normalization Instead, this kit is designed to distinguish types of negative results: a negative call for the target sequence and a positive call for the IPC indicate that no target sequence is present, and a negative call for the target sequence and negative call for the IPC suggest PCR inhibition.

Order Info

Note: This is not a quantitative kit and should not be used for normalization. Instead, this kit is designed to distinguish types of negative results: a negative call for the target sequence and a positive call for the IPC indicate that no target sequence

Specifications

• Content And Storage: • 1 ml 10X Exo IPC Mix
  • 120 μl 10X Exo IPC Block
  • 200 μl 50X Exo IPC DNA
  
  Store at -20°C to -25°C. Reagents may be stored at 2–4°C if they are to be consumed within one month.
• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Internal Probe Modification: TAMRA™ Quencher (3'), VIC™ (5')
• Product Line: TaqMan™
• Shipping Condition: Dry Ice
• No. of Reactions: 200 Reactions

• Format: Tube(s)
• PCR Method: qPCR
• Green Features: Sustainable packaging
• Label or Dye: VIC
• Product Type: Exogenous Internal Positive Control Reagent
• Sufficient For: 200 Reactions

For Research Use Only. Not for use in diagnostic procedures.