Applied Biosystems™ TaqMan™ Genotyping Master Mix
Optimized for end-point fluorescence detection in SNP genotyping applications.
Manufacturer: Applied Biosystems™ 4371353
- Distinct clusters and high call rates for unambiguous allelic discrimination
- Validated with TaqMan™ SNP Genotyping Assays
- Excellent pre- and post-PCR stability for high throughput setup and analysis
- Reaction size is based on a 50μL reaction volume
May be used for a variety of genotyping applications, including:
- Candidate gene studies
- Drug-target validation
- Disease association studies
- Population genetics
- Linkage mapping
- Agricultural applications
Exceptional Performance from an Optimized Mix (2X)
Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan™ Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include:
- AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure) - Automatic hot start enzyme designed to be active during thermal cycling and inactive at room temperature for easy reaction setup
- Optimized mix components provide excellent specificity for discrimination between alleles
- Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems™ real-time PCR instruments
- Single thermal cycling condition for consistent results with TaqMan™ Assays
Validated with TaqMan™ Genotyping Assays and Instruments:
- TaqMan™ Genotyping Master Mix has been tested across all types of TaqMan™ SNP Genotyping Assays: TaqMan™ Drug Metabolism Genotyping Assays, TaqMan™ SNP Genotyping Assays, and Custom TaqMan™ SNP Genotyping Assays
- In addition, the master mix is validated with the Applied Biosystems™ thermal cyclers and real-time PCR systems
High-Throughput Setup and Analysis:
- The combination of several low-cost thermal cyclers for PCR and a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable
- TaqMan™ Genotyping Master Mix further improves high-throughput setup and analysis with excellent room temperature stability both before and after PCR
- This provides the flexibility required to run long experiments, unattended overnight or over a weekend
Reliable Discrimination with Challenging Targets
- Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions
- The optimized components of TaqMan™ Genotyping Master Mix result in tight, well-separated clusters and more accurate allele-calls compared to other mixes, even for challenging targets
Setting the Industry Standard for Quality:
- Extensive analytical and functional tests are performed for each manufactured lot of TaqMan™ Genotyping Master Mix, including one TaqMan™ SNP Genotyping Assay and one TaqMan™ Drug Metabolism Genotyping Assay
- Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems™ Web site
Guaranteed minimum shelf life is 60 days.
|Supplied at 2X concentration. The mix is optimized for SNP genotyping using TaqMan™ probes and contains AmpliTaq Gold™ DNA Polymerase UP (Ultra Pure), dNTPs without dUTP, Passive Reference 1 and optimized mix components. This pack contains one 1 mL tube, sufficient for 40 reactions at the 50 µL total volume.
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
|AmpliTaq Gold DNA Polymerase|
|Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known)|
|Genotyping Master Mix|
|1 x 1 mL|
|SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation)|
|Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7|
|UP (Ultra Pure)|
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For Research Use Only. Not for use in diagnostic procedures.