Applied Biosystems™ TaqMan™ Microbe Detection Assay, pooled

Catalog No.	Quantity
A52083	250 μL

Catalog No. A52083 Supplier Applied Biosystems™ Supplier No. A52083

Description

Applied Biosystems TaqMan Microbe Detection assays are used for quantitative real-time PCR detection of microbial species.

Applied Biosystems TaqMan Microbe Detection assays are used for quantitative real-time PCR detection of microbial species. These assays consist of up to four pooled unlabeled PCR primers and TaqMan probes with a dye label (FAM) on the 5’ end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3’ end.

Features of TaqMan Microbe Detection assays include:
• Easy to use—just add DNA and master mix and run the qPCR—no melt curves required
• Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
• Sensitive—TaqMan assays are ideal for measuring low-abundance microbial targets
• Accurate—identify microbial targets with high accuracy of quantitation
• Extensive content—over 300 pre-designed microbe detection assays currently available
• Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for closely related microbial species and subtypes
• Fast delivery—assays shipped in as little as 1–2 days (see below)

Approximate ship time
• Inventoried: 1–2 days in North America*
• Made to order: 4–6 days in North America

All of our predesigned TaqMan gene expression assays are covered by the TaqMan Assays qPCR Guarantee.**

*Our most popular assays are stocked in North America for overnight delivery where possible. If an assay is out of stock, it may take an additional 3–5 business days for delivery.

**Subject to terms and conditions. For complete details, go to www.thermofisher.com/taqmanguarantee.

Specifications

• 3'Primer Modification: FAM
• Concentration: 20X
• 5'Primer Modification: MGB-NFQ
• Content And Storage: Store at -15 to -25°C.
  
  
• Detection Method: Primer-probe
• Format: Tube
• PCR Method: qPCR
• Product Line: TaqMan
• Quantity: 250 μL
• Shipping Condition: Dry Ice
• For Use With (Application): Pathogen Detection
• No. of Reactions: 250 Reactions

Frequently Asked Questions (FAQs)

What are the concentrations of the primers and probes for TaqMan microbial assays?

Our predesigned and custom TaqMan microbial 20X assays have a primer concentration of 900 nM and a probe concentration of 250 nM in the final 1X concentration.

For Research Use Only. Not for use in diagnostic procedures.