Applied Biosystems™ TaqMan™ MicroRNA Reverse Transcription Kit

Description

Provides the necessary components for 200 reactions of optimal performance in TaqMan™ MicroRNA Assays.

A highly specific kit that quantitates only mature miRNAs, not precursors. A sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is fast, simple and scalable 2-step quantitative RT-PCR assay that provides high-quality results in less than three hours.

Components of this kit are used with the RT primer provided with the TaqMan™ MicroRNA Assay to convert miRNA to cDNA.

Order Info

Shipping Conditions: Dry Ice

Specifications

• Concentration: 10X
• Content And Storage: Contains 10X RT buffer, dNTP mix w/dTTp (100 mM total), RNase inhibitor (20 U/μL), and MultiScribe™ RT enzyme (50 U/μL) for 1000 reactions. Store at -15 to -25°C.
• Detection Method: Primer-probe
• GC-Rich PCR Performance: High
• PCR Method: 2-step RT-qPCR
• Reaction Speed: Fast
• Optimal Reaction Temperature: 42°C
• Product Line: TaqMan™
• Product Type: MicroRNA Reverse Transcription Kit
• Quantity: 1,000 reactions
• Reverse Transcriptase: MultiScribe™
• Shipping Condition: Dry Ice
• Sufficient For: 1000 Reactions
• For Use With (Application): miRNA analysis
• Fidelity (vs. Taq): 10 X
• Final Product Type: cDNA
• No. of Reactions: 1000 Reactions
• Reaction Format: Separate Components
• Sample Type: RNA
• Size (Final Product): 200 bp or less

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.