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  7. Applied Biosystems™ TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR) 

Applied Biosystems™ TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)

Catalog No. 4488593
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Catalog No. No. of Reactions
44-885-93 200 Reactions
43-911-28 40 Reactions
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Catalog No. 44-885-93 Supplier Applied Biosystems™ Supplier No. 4488593
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Description

TaqMan™ PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample.

TaqMan™ PreAmp Master Mix lets you: • Analyze mRNA from precious samples such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded tissues (FFPE) • Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan™ Gene Expression assays • Analyze up to 100 gene expression targets with minimal hands-on time Preserves Equilibrium of Targets Without Preamplification Bias: In the past, preamplification kits and techniques often resulted in uneven amplification of targets and incorrect or biased data (as shown by low correlation coefficients between preamplified and unamplified samples). TaqMan™ PreAmp Master Mix amplifies with no bias and provides extremely high correlation between amplified and unamplified cDNA. Easy Workflow: With TaqMan™ PreAmp Master Mix, preamplification setup takes only a few minutes. After a one-time pooling of the TaqMan™ assays , preamplification setup (mixing the pooled assays with cDNA sample and TaqMan™ PreAmp Master Mix) takes only five additional minutes. Cycling takes 1.5 hours using a GeneAmp™ 9700 thermal cycler. For Research Use Only. Not for use in diagnostic procedures.

Order Info

Guaranteed minimum shelf life is 60 days.

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Contains one 5-mL bottle of 2X master mix.

Store at 2–8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Detection Method Primer-probe
Format Bottle
GC-Rich PCR Performance High
PCR Method qPCR
Reaction Speed Standard
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Product Line TaqMan
Product Type PreAmp Master Mix
Quantity 200 reactions
Sufficient For 200 Reactions
For Use With (Application) Pre-amplification
No. of Reactions 200 Reactions
Sample Type cDNA
Volume 5 mL
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For Research Use Only. Not for use in diagnostic procedures.

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