MilliporeSigma™ Tau, oligomeric, Mouse, Unlabeled, Clone: TOMA-1,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABN819

Catalog No. MABN819

Description

Microtubule-associated protein tau (UniProt P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as TAU, MAPT1, MTBTL) gene (Gene ID 4137) in human. In Alzheimer's disease (AD) pathology, accumulation of the microtubule-associated protein tau takes place primarily in the neurons. Tau accumulates in both the somatodendritic and axonal domains of neurons. Tau also accumulates in the soma as neurofibrillary tangles (NFTs). Cell death and synaptic lesions occur independently of NFT formation, and research indicates that NFT formation alone is insufficient for neurodegeneration, suggesting that soluble tau aggregates may be the more toxic and pathologically significant tau species. Tau oligomers are neurotoxic when applied extracellularly to cultured neuronal cells, and tau oligomers (but not fibrils) induce neurodegeneration and synaptic and mitochondrial dysfunction in vivo. Moreove, researchers are able to use tau oligomers as a reliable biomarker to differentiate AD brains from age-matched non-AD brains.

Specifications

• Antigen: Tau, oligomeric
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified mouse IgG2aκ in buffer containing PBS without preservatives.
• Gene Accession No.: P10636
• Immunogen: Recombinant human Tau-441 (Tau-F, Tau-4, 2N4R isoform) oligomers (Lasagna-Reeves, C. A., et al. (2012). FASEB J. 26(5):1946-1959).
• Quantity: 100 μg
• Research Discipline: Neuroscience
• Gene ID (Entrez): NP_058519.3
• Target Species: Human
• Form: Purified

• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Neutralization, Western Blot
• Clone: TOMA-1
• Conjugate: Unconjugated
• Gene: MAPT, TAU, MTBT1, MAPTL
• Host Species: Mouse
• Purification Method: Protein A Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone TOMA-1 (a.k.a. clone H12C10) is among more than 13 hybridomas derived from mice immunized with in vitro generated recombinant Tau-441 (Tau-F, Tau-4, 2N4R isoform) oligomers. These TOMAs are reported to specifically detect oligomeric tau without any significant reactivity toward monomeric tau, tau fibrils, Aβ oligomers, Aβ fibrils, or α-synuclein oligomers (Castillo-Carranza, D.L., et al. (2014). J. Neurosci. 34(12):4260-4272).
• Content And Storage: Stable for 1 year at -20°C from date of receipt.
• Isotype: IgG2a κ

For Research Use Only. Not for use in diagnostic procedures.