TBK1 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA534809

Catalog No. PIPA534809

Description

IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Nuclear factor kappa B (NF-KB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-KB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-KB is associated with IKB proteins in the cell cytoplasm, which inhibit NF-KB activity. Phosphorylation of I-KB by IKB kinase (IKK) complex leads to degradation of I-KB and activation of NF-KB. The IKK complex contains IKKa, IKKß, and IKKK. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-KB-activating kinase), and T2K. NAK/TBK1 activates IKKß through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-kB activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.

Specifications

• Antigen: TBK1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: TBK1
• Gene Alias: T2K, NF-kB-activating kinase, NF-kappa-B-activating kinase, serine/threonine-protein kinase TBK1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 29110
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.7 mg/mL
• Formulation: PBS with no preservative; pH 7
• Gene Accession No.: Q9UHD2
• Gene Symbols: TBK1
• Immunogen: Recombit fragment corresponding to a region within amino acids 169 and 416 of NAK (Uniprot IDNo.Q9UHD2)
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG