TCR β Chain Hamster anti-Mouse, Brilliant Violet 650, Clone: H57-597, BD Optibuild™

Armenian Hamster Monoclonal Antibody

Supplier:  BD Biosciences 742483

Catalog No. BDB742483

Description

The H57-597 antibody reacts with a common epitope of theβchain of the T-cell Receptor (TCR) complex onαβTCR-expressing thymocytes, peripheral T lymphocytes, NK1.1+ thymocytes, and NK-T cells of all mouse strains tested. It does not react withγδTCR-bearing T cells. In the fetal and adult thymus, the TCRβ-chain may form homodimers or pair with the pre-TCRα-chain on the surface of immature thymocytes before TCRα-chain expression. Plate-bound or soluble H57-597 antibody activatesαβTCR-bearing T cells, and plate-bound mAb can induce apoptotic death.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.

Specifications

• Antigen: TCR β Chain
• Clone: H57-597
• Conjugate: Brilliant Violet 650
• Gene Alias: Tcrb, TCRbeta, TCRβ, T cell receptor beta chain
• Immunogen: TCR affinity-purified from mouse T-cell hybridoma DO-11.10
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Armenian Hamster
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2 λ1