Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

TCR β Chain Hamster anti-Mouse, Brilliant Violet 650, Clone: H57-597, BD Optibuild™

Armenian Hamster Monoclonal Antibody

Manufacturer:  BD Biosciences 742483

Catalog No. BDB742483


Add to cart

Description

Description

The H57-597 antibody reacts with a common epitope of theβchain of the T-cell Receptor (TCR) complex onαβTCR-expressing thymocytes, peripheral T lymphocytes, NK1.1+ thymocytes, and NK-T cells of all mouse strains tested. It does not react withγδTCR-bearing T cells. In the fetal and adult thymus, the TCRβ-chain may form homodimers or pair with the pre-TCRα-chain on the surface of immature thymocytes before TCRα-chain expression. Plate-bound or soluble H57-597 antibody activatesαβTCR-bearing T cells, and plate-bound mAb can induce apoptotic death.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.
Specifications

Specifications

TCR β Chain
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
Tcrb, TCRbeta, TCRβ, T cell receptor beta chain
TCR affinity-purified from mouse T-cell hybridoma DO-11.10
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV650 under optimal conditions that minimize unconjugated dye and antibody.
RUO
Primary
Murine
H57-597
Brilliant Violet 650
Affinity Purified
Armenian Hamster
Armenian Hamster IgG2, λ1
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit