Invitrogen™ TCR DO11.10 Monoclonal Antibody (KJ1-26 (KJ126, KJI-26, KJI26)), PE-Cyanine7, eBioscience™

Catalog No.	Quantity
25-580-882	100 μg

Catalog No. 25-580-882 Supplier Invitrogen™ Supplier No. 25580882

Description

Mouse Monoclonal Antibody

The KJ1-26 anti-idiotypic monoclonal antibody binds the T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on any other T cell hybridomas tested.The TCR expressed on DO11.10 recognizes an ovalbumin peptide in the context of I-A^d major histocompatibility molecules.The use of DO11.10 and KJ1-26 have proven to be instrumental in describing many of the intricate details of T cell immunity. Applications Reported: This KJ1-26 (KJ126, KJI-26, KJI26) antibody has been reported for use in flow cytometric analysis. Applications Tested: This KJ1-26 (KJ126, KJI-26, KJI26) antibody has been tested by flow cytometric analysis of DO11 cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some...

TCR DO11 (DO11.10) is a receptor found on lymphocytes of DO11.10 transgenic mice and T-cell receptors of BALB/c-derived DO11.10 and DO11.10.24 T cell hybridomas. The DO11.10 TCR recognizes a C-terminal ovalbumin peptide (OVA 323-339) in the context of I-Ad major histocompatibility molecules. The use of antibodies against DO11.10 have proven to be instrumental in describing many of the intricate details of T cell immunity, including T cell activation, development, and function.

Specifications

• Antigen: TCR DO11.10
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: KJ1-26 (KJ126, KJI-26, KJI26)
• Concentration: 0.2 mg/mL
• Conjugate: PE-Cyanine7
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: Tcra
• Gene Accession No.: P01849
• Gene Alias: D011.10; T cell receptor alpha chain; Tcra; Tcralpha
• Gene Symbols: Tcra
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 21473
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ